Table 2. Description of Siberian Apricot candidate reference genes.
Genea | Homologous species | Homologous Locus | % homology | E vuale | Primers locationb |
ACT | Fragaria vesca subsp | XM_004294460.1 | 90% | 0 | N/F |
TUA | Fragaria vesca subsp | XM_004289661.1 | 91% | 0 | 3/4 |
TUB | Fragaria vesca subsp | XM_004294187.1 | 88% | 0 | 2/3 |
CYP | Fragaria vesca subsp | XM_004289796.1 | 87% | 2e-174 | N/F |
DNAj | Populus trichocarpa | XM_002316443.1 | 86% | 1e-121 | 2–3/4 |
ELFA | Fragaria vesca subsp | XM_004309835.1 | 92% | 0 | 1–2/2 |
GAPDH | Vitis vinifera | XM_002263109.2 | 84% | 0 | 9/10 |
RPL12 | Fragaria vesca subsp. | XM_004291528.1 | 87% | 6e-156 | N/F |
UBC | Fragaria vesca subsp. | XM_004287962.1 | 84% | 5e-162 | 4/5 |
UBQ | Fragaria vesca subsp | XM_004294293.1 | 87% | 0 | N/F |
ACT, TUA, TUB, CYP, DNAj, ELFA and UBQ were ESTs based on the other species reference gene sequence determined via BLASTN.
The prediction of exon, the previous digits indicated the site of primer forward and the back correspond to primer reverse. N/F meaning no intron or limited ESTs could not conjecture the intron.