Table 3. Reference genes and their primer sequences used for real-time PCR.
| Abbreviation | Gene name | Accession No. | Primer forward(F)/reverse(R) | Primer reverse | Amplicon size (bp) | Eb (%) | R2 b |
| ACT | Actin | CV049956.1 | ACATTGTTCTTAGTGGTGGGTC | AGATTCGTCATACTCTGCCTTT | 209 | 100.5±4.8 | 0.994 |
| TUA | Alpha-tubulin | CV046479.1 | TTGACATTGAGCGACCCACC | TCACATCCACATTCAGAGCACC | 108 | 100.5±2.8 | 0.997 |
| TUB | Beta-tubulin | CV045221.1 | CTTGACAATGAAGCCCTCTATGA | AGTAAGAGGAGCAAAGCCCAC | 219 | 104.6±5.7 | 0.995 |
| CYP | Cyclophilin | CV046015.1 | CAACGGATCTCAGTTCTTCGTCTGC | GACCCAACCTTCTCGATGTTCTTCA | 120 | 101.8±2.1 | 0.998 |
| DNAj | Chaperone protein dnaJ | CV052151.1 | GGTGGACACGACCCATTTGA | ACCTGACTTTGACCCTTTACCC | 219 | 105.1±4.7 | 0.998 |
| ELFA | Elongation factor 1-alpha | CV046439.1 | ACTGGAACCTCACAGGCTGAC | GGAGTAGTGGCATCCATCTTGTTA | 170 | 105.9±3.6 | 0.993 |
| F-box 27 | F-box protein 27 | EU836687.1 | CGTGGAGTGATTTGATTGGC | AAGTTTGGGTGGTGGAGGC | 106 | 99.8±5.5 | 0.997 |
| GAPDH | Glyceraldehyde-3-phosphate | JN786944.1 | ATGTCTTTCCGTGTTCCTACTGT | TTTCCCTCAGACTCCTCCTTG | 116 | 100.4±3.5 | 0.993 |
| RPL12 | Ribosomal protein L12 | U93168.1 | CGATCCCTCACAGGTCGTCG | TCCAGTCGTTGGCGGTCTCC | 149 | 99.2±3.3 | 0.990 |
| UBC | Ubiquitin-conjugating enzyme | AF008910.1 | GAGACCAGCAATAACCGTGAA | TCTTGTACTCCGTGGCATCCT | 128 | 99.2±4.2 | 0.997 |
| UBQ | Polyubiquitin | CB821710.1 | CTCTGACTGGCAAGACCATAACA | CCACGGAGACGAAGGACAA | 205 | 102.0±1.9 | 0.998 |
b
PCR efficiency (E) and correlation coefficients (R2) were calculated by LinRegPCR method.