Figure 5. Quantification of intracellular Cd²⁺ and Zn²⁺.

MDBK cells were treated with 10 μM (A) or 50 μM (B) Cd²⁺, alone or in combination with Zn²⁺ (0, 10, or 50 μM). At the indicated time points, the intracellular levels of Zn²⁺ and Cd²⁺ (ng/1×10⁴ cells) were determined by ICP-MS, as described in the Materials and Methods section. The results represent the mean ± SD (n = 4). *P<0.05, compared to the cells without Zn²⁺ supplementation.