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. 2014 Aug 8;9(8):e104205. doi: 10.1371/journal.pone.0104205

Figure 1. The sera obtained from the patients with t-CIDP, MADSAM and DADS disrupted the BNB.

Figure 1

(A) – (D) Effects of the sera obtained from patients with three different phenotypes of chronic inflammatory demyelinating polyneuropathy (CIDP) on the protein levels of claudin-5 and occludin in the FH-BNBs, as determined using a Western blot analysis. The cells were exposed to sera from either patients with typical CIDP (t-CIDP) (A), multifocal acquired demyelinating sensory and motor neuropathy (MADSAM) (B) or distal acquired demyelinating symmetric neuropathy (DADS) (C) or healthy volunteers (D). (E) The sera obtained from the patients with t-CIDP, MADSAM neuropathy and DADS neuropathy decreased the protein ratio of claudin-5 to actin proteins in the FH-BNBs compared to that observed following exposure to the sera from the healthy volunteers. The decrease in the claudin-5 levels in the FH-BNBs was greater after incubation with the sera obtained from the t-CIDP patients than after that with the sera from the patients with MADSAM and DADS. (F) There were no significant differences between the patients with the three different phenotypes of CIDP and the healthy controls regarding the occludin protein levels in the FH-BNBs. (G) The effects of the sera on the transendothelial electrical resistance (TEER) values in the FH-BNBs were also evaluated. Adding sera obtained from the patients with t-CIDP, MADSAM neuropathy or DADS neuropathy resulted in decreased TEER values in the FH-BNBs in comparison with that observed in the cells treated with the sera obtained from the healthy volunteers. Markedly decreased TEER values in FH-BNBs were also observed in the FH-BNBs following incubation with the sera obtained from the t-CIDP patients compared to that noted in the cells incubated with sera from patients with MADSAM or DADS neuropathy. The TEER values were decreased following exposure to the sera obtained from the patients with DADS neuropathy compared to that observed after exposure to the sera obtained from the patients with MADSAM neuropathy. The bars indicate the mean level in each group. Control: non-conditioned DMEM containing 20% FBS. t-CIDP: conditioned medium with 10% sera obtained from patients with t-CIDP diluted with non-conditioned DMEM containing 10% FBS. MADSAM: conditioned medium with 10% sera obtained from patients with MADSAM diluted with non-conditioned DMEM containing 10% FBS. DADS: conditioned medium with 10% sera obtained from patients with DADS diluted with non-conditioned DMEM containing 10% FBS. Normal: conditioned medium with 10% sera obtained from a healthy volunteer diluted with non-conditioned medium of DMEM containing 10% FBS.