Table 1.
Details of seven polymorphic microsatellite loci characterized in 100 A. femoralis individuals from French Guiana; k (number of alleles observed), AT (annealing temperature), Ho (observed heterozygosity), He (expected heterozygosity), PIC (mean polymorphic information content), PHWE (probability for deviation from Hardy–Weinberg equilibrium), P Null(van Oosterhout estimate for the frequency of null alleles at each locus).
| Locus | Repeat motif | Dye and primer sequence (5′–3′) | k | Size range of amplified product (bp) | AT (°C) | H O | HE | PIC | P HWE | P Null | GenBank accession no |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Afem04 | (TAGA)7 | F: HEX-GAGACGCCTGTTATAGATGGTG R: TTAAAATGCACCGACACTGG |
21 | 248–336 | 56 | 0.95 | 0.919 | 0.908 | NS | −0.0213 | KF021569 |
| Afem20 | (CA)15 | F: TET-TCCTAAGCCAGAGGAAGCTG R: ACAATCACATGCACCGAGTC |
6 | 139–151 | 56 | 0.73 | 0.72 | 0.669 | NS | −0.0083 | KF021570 |
| Afem22 | (AGAC)9(AGAT)14 | F: NED-ACCGTGGAGTGGTTGATGAG R: CCTGCCAAGGATTGATAAGC |
23 | 162–258 | 56 | 0.949 | 0.938 | 0.929 | NS | −0.0094 | KF021571 |
| Afem23 | (TAGA)8 | F: FAM-AGGCAGAGATTGCAGAGAATG R: GTGGCCATCTCCTGTTCATC |
9 | 219–279 | 56 | 0.273 | 0.66 | 0.596 | ** | 0.2689 | KF021572 |
| Afem24 | (ATAG)13 | F: NED-AAAGTAGGGTCGCAGCACTC R: AGGTCAAGTCGGATGGTTTG |
25 | 177–293 | 56 | 0.878 | 0.943 | 0.935 | NS | 0.0323 | KF021573 |
| Afem25 | (AC)15 | F: HEX-GTAATCCCCCAATCCTGGTC R: GATCCCGGCATCGTTAAG |
27 | 168–286 | 61 | 0.878 | 0.92 | 0.909 | NS | 0.0218 | KF021574 |
| Afem27 | (AC)12 | F: FAM-AACGCAAACACACCCTTAGC R: ATCACTTGACATGGAATGACC |
24 | 163–245 | 56 | 0,9 | 0,947 | 0.938 | NS | 0.0214 | KF021575 |