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. 2014 Aug 8;55(8):4913–4921. doi: 10.1167/iovs.13-13346

Figure 4.

Figure 4

Fluorescent labeling in retinal wholemounts after intravitreal injection of NMDA and Zn-DPA 480. For all experimental groups, intravitreal injection of Zn-DPA 480 was performed 1 hour before euthanasia. At 4 hours after injection of NMDA, Zn-DPA 480–positive cells in the RGC layer were noted. At 48 hours after injection of NMDA, mild Zn-DPA 480 labeling showing negative DAPI nuclear counterstaining was detected (arrows). No positive Zn-DPA 480 cells were noted 4 hours after PBS injection (no NMDA).