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. 2014 Aug 8;55(8):4913–4921. doi: 10.1167/iovs.13-13346

Figure 6.

Figure 6

(A) Confocal laser micrographs showed RGCs with double labeling of Zn-DPA 480 and TUNEL after NMDA injection. There were Zn-DPA 480–positive cells (green) in the RGC layer (arrowheads) but no TUNEL-positive cells were noted in the retina at 2 hours postinjection (top). At 24 hours postinjection, TUNEL-positive cells were observed in both the RGC layer and inner nuclear layer, whereas colocalization of Zn-DPA 480 (green) and TUNEL (red) predominantly appeared in the RGC layer (bottom; arrows) but not in the inner nuclear layer. DAPI was used as nuclear counterstaining (blue). (B) High magnification of RGC layer cells at 4 hours after NMDA injection showed colocalization of Zn-DPA 480 (green) and TUNEL (red; arrows). However, the RGC layer cells with condensed DAPI nuclear counterstaining (blue) were TUNEL and Zn-DPA 480 negative (arrowheads).