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. 2014 Aug 8;55(8):4913–4921. doi: 10.1167/iovs.13-13346

Figure 7.

Figure 7

Cellular localization of Zn-DPA 480 in retina at 24 hours after NMDA injection. (A–D) Rbpms immunohistochemistry (green = Zn-DPA 480; red = Rbpms; blue = DAPI). There was colocalization of Zn-DPA 480 (A) and Rbpms (B) in the cells in the RGC layer (arrows). Very few Zn-DPA 480–positive cells were noted in the inner part of inner nuclear layer but were Rbpms negative (*). (E–H) Vimentin immunohistochemistry (green = Zn-DPA 480; red = vimentin; blue = DAPI). Zn-DPA 480–positive cells (E) were vimentin (F) immuno-negative (double arrowheads). (I–L) III β-tubulin immunohistochemistry (green = Zn-DPA 480; red = III β-tubulin; blue = DAPI). Zn-DPA 480–positive cells (I) were III β-tubulin (J) immuno-positive (arrows). (M) Quantitative analysis of Zn-DPA 480 labeling with III β-tubulin and vimentin immunohistochemistry. More than 50% of Zn-DPA 480–positive cells were labeled by a commonly used RGC marker, beta-tubulin, whereas a few Zn-DPA 480–positive cells were labeled by Müller cell marker, vimentin. There was no double labeling of Zn-DPA 480 and III β-tubulin or vimentin in the control (no NMDA) retina (data not shown). n = 4. Error bars: SD.