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. Author manuscript; available in PMC: 2015 Feb 7.
Published in final edited form as: Nature. 2014 Jun 29;512(7512):69–73. doi: 10.1038/nature13322

Figure 2. Hyperactivation of the Nlrp3, but not the Nlrc4 and AIM2 inflammasomes, in A20-deficient macrophages.

Figure 2

a-h, BMDMs were stimulated as described in Methods. Lysates were immunoblotted for caspase-1 (a, c, d) and supernatants analyzed for IL-1β (b, e, f) and LDH (g, h). i-n, BMDMs were treated as described in Methods. Lysates were immunoblotted for caspase-1 (i, k) and supernatants analyzed for LDH (j, l) and IL-1β (m, n). Black arrow, procaspase-1; white arrow, p20. Data represent mean ± s.d. of 1 out of 3 biological replicates, with 3 technical replicates each (*P < 0.05; ***P < 0.001; Student’s t-test).