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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: FEMS Yeast Res. 2014 Jun 9;14(5):762–775. doi: 10.1111/1567-1364.12165

Figure 6. Secretion of degradative lipases.

Figure 6

Cells from overnight cultures were transferred to medium to induce secretion of lipases. The resulting supernatants were used in a turbidimetric kinetic assay for esterases described by von Tigerstrom and Stelmaschuck (von Tigerstrom & Stelmaschuk, 1989). Optical densities (λ=500nm) measured precipitates of calcium salts of fatty acid which results from the hydrolysis of the substrate, Tween 20. The rate of increase in optical density is proportional to the concentration of lipase present in the supernatant. Data presented is the average of three independent experiments and error bars indicate standard deviation values.