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Cells at a concentration of 5×106 cells ml−1 were grown in buffered RPMI-1640 at 37 °C with shaking at 200rpm. Filamentation was followed at hourly intervals by DIC microscopy. Representative DIC images using a 63X oil objective, taken at T=5 hr, are shown. Formation of the bulbous tip in the tetR-SSO2 strain grown in the presence of DOX is typical (arrow). Scale bar shown represents 5 μm.
