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. Author manuscript; available in PMC: 2015 Aug 5.
Published in final edited form as: Cell Metab. 2014 Jun 19;20(2):241–252. doi: 10.1016/j.cmet.2014.05.012

Figure 4. Drosophila Sdhaf4 is required for SdhA and SdhB stability.

Figure 4

(A) Mitochondrial extracts from either w1118 controls (dSdhaf4+) or dSdhaf41/2 mutants (dSdhaf4) were fractionated by BN-PAGE and analyzed by immunoblotting to detect Atpα (Complex V) or SdhB (Complex II). (B) Total protein from mitochondria isolated from either control or dSdhaf4 mutant flies was resolved using SDS-PAGE, followed by immunoblotting to detect SdhA, SdhB or Atpα. (C) Flies carrying either control or dSdhaf4 mutant chromosomes over a balancer chromosome were crossed to flies carrying a strong loss-of-function allele for either SdhA or Cytochrome c oxidase Va subunit (CoVa) over a balancer. The resulting progeny were scored for the absence or presence of the marker linked to the balancer chromosome. The proportion of the expected progeny that eclosed, based on Mendelian ratios of the indicated genotypes, is shown. (±SEM. N = number of progeny assayed from each cross. ***p <0.001 (Student’s t test).