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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Arch Pharm Res. 2013 Oct 8;37(12):1607–1616. doi: 10.1007/s12272-013-0257-5

Fig. 1.

Fig. 1

Negative-stain TEM micrographs of influenza vaccine particles. Inactivated A/PR/8/34 (H1N1) influenza virus vaccines. a split vaccine particles and b whole inactivated virus (WIV) vaccine particles. Vaccines incubated in iso-osmotic sucrose medium (pH 7.0, 4 °C) were stained with phosphotungstic acid (2 %, pH 7.0) and allowed to dry under ambient conditions before imaging. Inset at the left bottom in a shows magnified split vaccine image. Vaccine particle size was determined by TEM image analysis for split vaccine particles (30 ± 6 nm in diameter, n = 30) and WIV vaccine particles (87 ± 17 nm in diameter, n = 80)