Table 3.
Immunohistochemical panel
| Antigen/antibody/clone | Company | Dilution | Antigen recovery | Number of cases with positive reactions | Reaction pattern |
|---|---|---|---|---|---|
| Cytokeratin (AE1/AE3:M3515) | Dako, Carpinteria, CA | 1:40 | CC1, 30 min | 14/14 (100 %) | S, D, C |
| CK7 (OV-TL-12/30) | Dako | 1:200 | CC1, 30 min | 7/14 (50 %) | S, D-F, C |
| CK5/6 (D5/16 B4) | Dako | 1:25 | E2, 20 min | 12/14 (86 %) | S, D, C |
| Epithelial membrane antigen (EMA)(E29) | Ventana Medical Systems, Tucson, AZ | Neat | CC1, 30 min | 13/14 (93 %) | S, F, C, L |
| CK903 (34ßE12; 1/5/10/14) | Enzo Life Sciences, Farmingdale, NY | Neat | n/a | 11/14 (86 %) | S, D, C |
| CEAm (CLO1-cea ab-1) | Lab Vision/NeoMarkers, Fremont, CA | 1:250 | CC1, 30 min | 13/14 (93 %) | S, L, C |
| p63 (7jul) | Leica Microsystems, Buffalo Grove, IL | 1:40 | E2, 30 min | 12/14 (86 %) | S, D, N |
| p53 (DO-7) | Dako | Neat | CC1, 30 min | 15/18 (83 %) | S, N, 1–90 % |
| p16INK4a (E6H4) | MTM Laboratories | Neat | CC1, 30 min | 5/20 (25 %) | S, F-D, N&Ca |
| CD56 (123C3.D5) | Lab Vision/NeoMarkers, Fremont, CA | Neat | CC1, 30 min | 2/14 (14 %) | S, D, C |
| Synaptophysin | Ventana | Neat | CC1, 30 min | 0/14 (0 %) | n/a |
| EBER (EBER1 DNP) | Ventana (BenchMark) | n/a | n/a | 0/14 (0 %) | n/a |
| Ki-67 (MIB-1) | Dako | 1:100 | CC1, 30 min | 2–90 % | S, N |
| HPV (ISH)(Y1404) | Dako | n/a | n/a | 5/19 (26 %) | N, dot/diffuse |
mm mouse monoclonal, rp rabbit polyclonal, S Strong, D Diffuse, F Focal, C Cytoplasmic, N Nuclear, L Luminal, n/a not applicable
aReactions ranged from 20 % to all cells. Only >70 % strong, diffuse, nuclear and cytoplasmic reactions were considered positive