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. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: Nature. 2014 May 7;509(7500):337–341. doi: 10.1038/nature13309

Figure 2.

Figure 2

Analysis of cardiac cells from Kit+/Cre × R-GFP mice. a, b, c, d, e, f, g, Immunofluorescent images of heart histological sections from Kit+/Cre × R-GFP mice at 4 weeks of age stained with eGFP antibody (green), nuclei in blue and either CD31, von Willebrand factor (vWF), CD34, CD3, CD45, vimentin or smooth muscle α-actin (αSMA) in red. Arrows show cells with overlap in staining. h, i, FACS plot showing lineage markers of heart isolated c-kit derived eGFP+ cells for CD45 (h) and CD31 (i) (representative of n=6 for CD45 at 4 weeks of age, and n=3 for CD31 at 12 weeks of age). j, Immunofluorescent image from heart histological section of a Kit+/Cre × R-GFP mouse at 4 weeks for eGFP fluorescence (green), CD31 antibody staining (blue) and NG2 antibody staining (red). Right panel shows composite with transmitted light.