Table 1.
Primers used to amplify and sequence the complete BATV S, M, and L segments
| Segment primer | Sequence (5′ to 3′) | Amplicon size (nt) |
|---|---|---|
| S segment primer |
|
|
| BATAS1F |
5′-GAATTCAATGATGTCGCTGCT-3′ |
818 |
| BATAS1R |
5′-CAATTTGCTGTGCTCTTTCTG-3′ |
|
| 3′S-Inner Primer |
5′-CTAGCAAAGTTTGGCATCAGC-3′ |
|
| 3′S-Outer Primer |
5′-GGGTGGAAGAAGACAAATGTG-3′ |
|
| 5′S-Inner Primer |
5′-GGGTCAAAAGTACTGCTGGTG-3′ |
|
| 5′S-Outer Primer |
5′-GTGTAGATACGCTTAAAGTTA-3′ |
|
| M segment primer |
|
|
| BATAM1F |
5′-GATGTTGCTACTTCTTGTCTT-3′ |
2013 |
| BATAM1R |
5′-AGTTTTGTAATACCAGTTGTG-3′ |
|
| BATAM2F |
5′-CAACTGAGTCACTCTTTTATG-3′ |
1973 |
| BATAM2R |
5′-CATAATCTTGTTTTGTGGAGG-3′ |
|
| BATAM3F |
5′-CTGCAGTTTTAAGATTGTTTC-3′ |
480 |
| BATAM3R |
5′-TGTTTTATTTCCTGTAGGTAC-3′ |
|
| 3′M-Inner Primer |
5′-GTACCTACAGGAAATAAAACA-3′ |
|
| 3′M-Outer Primer |
5′-TGATGTTTAAACTTAGAGATG-3′ |
|
| 5′M-Inner Primer |
5′-GAGGCAAAATTCTGATATAGC-3 |
|
| 5′M-Outer Primer |
5′-ATTTTTTTCATATATTATTTC-3′ |
|
| L segment primer |
|
|
| BATAL1F |
5′-AAAAATGGATGATCAGATGTA-3′ |
2025 |
| BATAL1R |
5′-TTCTCTGCTATATAATCTTTG-3′ |
|
| BATAL2F |
5′-TCGATATATGATAATGAATTC-3′ |
2021 |
| BATAL2R |
5′-CTAAACCAACTAAAGCTATCA-3′ |
|
| BATAL3F |
5′-TTAAGAGTCGTCATGATATAC-3′ |
1952 |
| BATAL3R |
5′-TGATAATACAACAGGACAGAC-3′ |
|
| BATAL4F |
5′-TAAAAAGAAATGAAGAAGGAC-3′ |
945 |
| BATAL4R |
5′-CTTAGAAAAAGGTGAACATGG-3′ |
|
| 3′L-Inner Primer |
5′-CAACTGGACCAAGATGCTAAA-3′ |
|
| 3′L-Outer Primer |
5′-CTGCCTTCTACATAAATACAG-3′ |
|
| 5′L-Inner Primer |
5′-GTTCCAACTGATACTTTATAG-3′ |
|
| 5′L-Outer Primer |
5′-GTATTTTTTATAAGTAATCTC-3′ |
|
| P1 |
5′-ATAGAATCAGCAATAGCAAGC-3′ |
550 |
| P2 | 5′-ATGATGATCTGTAACCTCTAA-3′ |