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Western analysis of brush border membrane association of the proteins under study. Western blots for BBM (left lanes) and cellular homogenate (HG) (right lanes) for ezrin, NHE3, NHERF1, NHERF2, NHERF3, flotillin2 and rab5 (three independent preparations from three mice). NHERF1 and NHERF2, albeit not transmembrane proteins, show a very strong membrane association, ezrin and NHERF3 to a lesser degree, and rab5 not at all.
