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Membrane raft association of NHE3 and NHERFs is altered by a change in lipid composition of BBM. A) In the ASM KO mice, NHE3 was distributed into rafts and non-rafts, but there was a pronounced shift of both flotillin2 and NHE3 towards even lighter density OptiPrep fractions which we denote as “lighter rafts” (L-rafts). B) Quantitative analysis showed that there was a twofold increase in NHE3 pool in rafts in ASM KO, 50% of which was localized in L-rafts (p<0.05). n=3.
