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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: J Immunol. 2014 May 12;192(12):6062–6070. doi: 10.4049/jimmunol.1303348

Figure 3. CCR7 and CXCR4 signaling in Elmo1−/− T cells.

Figure 3

(A) CXCR4 and CCR7 surface levels on splenic lymphocyte populations from WT and Elmo1−/− mice were determined by flow cytometry. One representative experiment of four is shown. (B, C) CD4+ T cells isolated from WT and Elmo1−/− spleens were stimulated 15 seconds with 250ng/mL CXCL12 or CCL19 and lysed. (B) Lysates were analyzed for Rac-GTP levels by GST-PAK pulldown and anti-Rac IB. Below, graph showing Rac-GTP levels normalized to total Rac and expressed as relative to medium control for n = 4 mice/genotype ±SEM. (C) phospho-Ser473-AKT and phospho-Thr202/Tyr204-ERK1/2 levels were determined by IB. Blots were stripped and re-probed for total AKT and ERK1/2. One representative experiment of three is shown.