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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: J Immunol. 2014 May 12;192(12):6062–6070. doi: 10.4049/jimmunol.1303348

Figure 5. Elmo1-dependent CD4+ T cell migration requires interaction with Dock2.

Figure 5

(A) Lysates from 293T cells transfected with vector, Dock2-Flag, Elmo1-Flag, or mutant Elmo1T629-Flag were IP'd with anti-Dock2 and analyzed by IB with anti-Flag. One representative experiment of three is shown. Relative molecular weights (kDa) are indicated to right. (B) Splenic CD4+ T cells from WT and Elmo1−/− mice were nucleofected with GFP plasmid along with either empty vector, Elmo1, Elmo1T629 or Elmo2 plasmids and assayed for transwell migration to CXCL12 (50ng/mL). The number of GFP+ cells migrating to the lower chamber was determined by flow cytometry and normalized to the total number of GFP+ cells for each transfected population and used to calculate the percent migration for each transfected populations (n = 3, ±SEM).