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. Author manuscript; available in PMC: 2015 Aug 7.
Published in final edited form as: Mol Cell. 2014 Jul 4;55(3):372–382. doi: 10.1016/j.molcel.2014.06.004

Figure 1. Identification of Blnc1 as an inducible lncRNA in brown fat.

Figure 1

(A) Venn diagrams of protein-coding (left) and lncRNA (right) genes induced during brown adipocyte differentiation (BAC d7/d0), enriched in BAT vs. eWAT, and induced in eWAT by CL-316,243 (CL/veh).

(B) qPCR analyses of gene expression during BAC differentiation.

(C) qPCR analyses of Blnc1 expression in adipose tissues and differentiated adipocytes.

(D) PhyloCSF analysis of Blnc1, Paqr9 and Ucp1.

(E) In vitro transcription/translation assay using luciferase (Luc) and Blnc1 constructs. Shown are 35S autoradiograph (left) and Ponceau S stained blot (right).

(F) Immunoblotting and qPCR analyses of cytosolic (Cyt) and nuclear (Nuc) fractions. Data represent mean ± sd. *p<0.05 eWAT vs. BAT; 3T3-L1 vs. BAC (C); and Cyt vs. Nuc (F). (See also Figure S1 and S2)