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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2014 May 9;1840(9):2878–2885. doi: 10.1016/j.bbagen.2014.05.004

Fig. 1.

Fig. 1

Hxt1 protein levels are posttranslationally downregulated in response to glucose starvation. (A) Western blotting analysis of the expression levels of Hxt1-GFP in the plasma membrane-enriched fraction. Yeast cells (WT) expressing Hxt1-GFP were grown in SC-2% glucose medium to mid log phase (O.D600nm = 1.2–1.5) and equal amounts of cells were shifted to SC medium containing different glucose concentrations. Membrane fractions were immunoblotted with anti-GFP antibody (top panel), and the intensity of each band on the blot was quantified by densitometric scanning (bottom panel). (B) Yeast cells (WT) expressing Hxt1-GFP were grown in SC-2% glucose (Glu) medium to mid log phase and equal amounts of cells were shifted to SC-2% galactose medium and incubated for indicated times. Membrane-enriched fractions were immunoblotted with anti-GFP antibody. (C) Determination of the half-life of Hxt1-GFP protein. The Western blot images in (B) were scanned and the half-life was determined, as described in the materials and methods.