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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2014 May 9;1840(9):2878–2885. doi: 10.1016/j.bbagen.2014.05.004

Fig. 5.

Fig. 5

K12 and K39 serve as putative ubiquitin-acceptor lysine residues. Yeast cells (WT) expressing indicated Hxt1-GFP proteins were grown as described in Fig. 2A. Plasma membrane fractions were immunoblotted with anti-GFP antibody (A), and subcellular localization of Hxt1-GFP (Wt, K12A and K39A) was analyzed by confocal microscopy (B).