Figure 3.
The CarD-DNA interaction is dispensable for resistance to killing by oxidative stress. Log-phase M. smegmatis strains expressing CarDWT, CarDR25E, CarDR47E, CarDW85A, CarDK90A, CarDK125A, or CarDK125E in LB were treated for 1 hr with 25 mM H2O2. After treatment, dilutions were plated on LB and the surviving CFUs were counted. Survival of each replicate is graphed as a ratio of CFU in treated cultures to that in untreated cultures along with the mean ± SEM of the ratios for each set of replicates. Each sample is represented by a black circle.