(A) K5-NICD breeding scheme and experimental plan.
(B-D) Representative IF images demonstrate differences in the repairing tracheas from the Wt uninjured, K5-NICD uninjured and treated with RU486, Wt (vehicle treated) 48 hpi, Wt DBZ treated 48 hpi and K5-NICD (with Ru486) 48 hpi. BrdU was examined for proliferation and acetylated β-tubulin, CC10 and PIgR were used as markers of differentiated cells.
(E) and (F) Bar graphs representing the total cell counts and proliferating cells seen under the in vivo experimental conditions. Data are presented as mean ± SEM; (n = 7), * P < 0.05 and ** P < 0.01.
See also Figure S6.