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. 2014 May 27;8(3):249–269. doi: 10.1007/s11693-014-9145-7

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Fig. 2 — Fission of buds in liposomes with liquid-disordered phase membranes. a Fission of a bud at the liquid-ordered/liquid-disordered separation line upon heating from (i) to (ii). Vesicle composition 0.615:0.135:0.25 SM:DOPC:cholesterol. phase was imaged in blue by incorporating small amount of a Dil dye, phase was imaged in red by incorporating a small amount of N-lissamine rhodamine dipalmitoylphosphoethanolamine. Scale bar 5 . b Fission of vesicle due to an encapsulation of an ATPS system. 0.35:0.35:0.3 POPC:DPPC:cholesterol vesicle encapsulating a PEG:dextran ATPS. Osmotic pressure increases form left to right. Blue indicates lectin SBA Alexa 647 (dextran phase); red indicates domain lipid (DOPE-rhodamine); and green indicates domain through streptavidin-Alexa488 (bound to the lipid DSPE-PEG-2K-biotin). Scale bar 10 microns. a Modified with permissions from reference Baumgart et al. (2003) (2003) Nature Publishing Group. b Modified with permission from reference Andes-Koback and Keating (2011) (2011) American Chemical Society. (Color figure online)

Inline graphic — Fission of buds in liposomes with liquid-disordered phase membranes. a Fission of a bud at the liquid-ordered/liquid-disordered separation line upon heating from (i) to (ii). Vesicle composition 0.615:0.135:0.25 SM:DOPC:cholesterol. phase was imaged in blue by incorporating small amount of a Dil dye, phase was imaged in red by incorporating a small amount of N-lissamine rhodamine dipalmitoylphosphoethanolamine. Scale bar 5 . b Fission of vesicle due to an encapsulation of an ATPS system. 0.35:0.35:0.3 POPC:DPPC:cholesterol vesicle encapsulating a PEG:dextran ATPS. Osmotic pressure increases form left to right. Blue indicates lectin SBA Alexa 647 (dextran phase); red indicates domain lipid (DOPE-rhodamine); and green indicates domain through streptavidin-Alexa488 (bound to the lipid DSPE-PEG-2K-biotin). Scale bar 10 microns. a Modified with permissions from reference Baumgart et al. (2003) (2003) Nature Publishing Group. b Modified with permission from reference Andes-Koback and Keating (2011) (2011) American Chemical Society. (Color figure online)