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Assay for IL-10-expressing cells in LNs. Flow cytometric analysis of popliteal LNs on day 7 after EAU immunization showed that the percentages of IL-10+CD19+ (a) and IL-10+B220+CD19+ cells (b) were significantly increased in mice treated with hMSCs, compared to mice without EAU or BSS-treated EAU mice. Data are presented in mean ± SEM. n = 5 in each group.
