Figure 7.

AEA increased intracellular SOD and ameliorated GSH/GSSG ratio. The cells were divided into six groups, Control: cells cultured in drug-free medium; AEA: cells exposed to 10 μM AEA for 3 h; H₂O₂: cells exposed to 200 μM H₂O₂ for 3 h; AEA + H₂O₂: cells exposed to 10 μM AEA plus 200 μM H₂O₂ for 3 h; AM251 + AEA + H₂O₂: cells exposed to 10 μM AEA plus 10 μM CB1 antagonist AM251 in the presence of 200 μM H₂O₂ for 3 h; AM251 + H₂O₂: cells exposed to 10 μM AM251 plus 200 μM H₂O₂ for 3 h. The intracellular SOD, GSH, and GSSG levels were assessed by the corresponding reagent kit, and the GSH/GSSG ratio was calculated according to the GSH and GSSG levels. (a) Intracellular SOD level. (b) Intracellular GSH level. (c) Intracellular GSSG level. (d) Intracellular GSH/GSSG ratio. Results are expressed as means ± SD (n = 6). *P < 0.05 versus control (no H₂O₂, no AEA, and no AM251), ^# P < 0.05 versus the cells exposed to H₂O₂ alone, and ^∧ P < 0.05 versus the cells exposed to AEA plus H₂O₂.