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. 2014 Jul 20;2014:652680. doi: 10.1155/2014/652680

Figure 5.

Figure 5

Real-time PCR analysis of mRNA expression of NF-κB-dependent proinflammatory markers in the livers of HFD-fed hamsters receiving 8 weeks of treatment with ruscogenin (3.0 mg/kg/day). The mRNA expressions of the inflammatory cytokines and NF-κB were normalized to an internal control (β-actin). Animals not receiving any treatment were given the same volume of vehicle used to dissolve ruscogenin. Similar results were obtained with an additional 4 replications. Data were expressed as the mean with SEM (n = 5 per group) in each column. a P < 0.01 and b P < 0.01 compared to vehicle-treated RCD-fed hamsters. c P < 0.05 and d P < 0.01 compared to the values of vehicle-treated HFD-fed hamsters in each group, respectively.