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SMCs fail to induce OT-II CD4+ T cell activation. Expression of CD69, CD25, and CD44 on OT-II CD4+ T cells cocultured with OVA- or OVA323–339 peptide-treated SMCs or OVA-treated DCs (used as positive control). Results are expressed as mean ± SEM from three separate experiments run in triplicate. ***P < 0.001 versus OT-II CD4+ T cells alone at 24 h; °°P < 0.01, °°°P < 0.001 versus OT-II CD4+ T cells alone at 48 h; ## P < 0.01 and ### P < 0.001 versus OT-II CD4+ T cells alone at 72 h.
