Skip to main content
. Author manuscript; available in PMC: 2014 Aug 10.
Published in final edited form as: J Am Coll Cardiol. 2011 May 24;57(21):2181–2189. doi: 10.1016/j.jacc.2011.01.024

Figure 5. Effect of nitrate supplementation on DOX-induced inhibition of mitochondrial complex I.

Figure 5

Graph A shows that DOX treatment decreased the rate of glutamate + malate oxidation (complex I substrate; Mean±SEM; * P<0.05 versus Saline; n=6 per group), but the succinate (complex II substrate) oxidation was not altered in the isolated mouse cardiac mitochondria. Nitrate supplementation completely prevented the impaired complex I-dependent oxidative rate. Graph B indicates that DOX treatment decreased the activities of complex I and NADH dehydrogenase in the heart tissue homogenate (Mean±SEM; * P<0.05 versus Saline; n=5 per group). Nitrate treatment maintained completely the complex I activity, whereas it only partially preserved NADH dehydrogenase activity († P<0.05 versus DOX).