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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Matrix Biol. 2014 Apr 13;0:28–38. doi: 10.1016/j.matbio.2014.03.005

Figure 1.

Figure 1

Identification and isolation of Sca1high ASCs from iWAT and eWAT. (A) Immunofluorescent detection of Sca1high cells in inguinal and epididymal white adipose tissues (iWAT and eWAT) isolated from 6-week-old C57/BL6 male mice. Sca1 (red), type IV collagen (green), and nucleus (blue). Scale = 100 μm. Representative figures, N=3. (B) Flow cytometry analysis of Sca1(+)F4/80(−) cells in iWAT- and eWAT-derived VSCs. Representative data, n ≥ 3. (C) The isolation of Sca1high ASCs from iWAT and eWAT using MACS, shown as mean ± SD. N=4. (D) The mRNA expression of Sca1 in the Sca1high ASCs isolated from iWAT and eWAT using MACS. N=4. Mean ± SEM. * P < 0.05 (E) The expression of SCA1 protein on the plasma membrane of Sca1high ASCs isolated from iWAT (red). Nucleus (blue); scale = 10 μm.