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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2014 Mar 22;0:473–480. doi: 10.1016/j.jsbmb.2014.03.004

Figure 1. Deletion mutagenesis analysis of AR nuclear import and export in COS1 cells upon androgen manipulation.

Figure 1

(A) Structures of GFP fusion protein constructs: GFP-AR, GFP-DBDH-LBD, and GFP-LBD. (B) COS1 cells were transiently transfected with GFP-AR, GFP-DBDH-LBD and GFP-LBD cultured in androgen-free conditions. The subcellular signal distribution was visualized by fluorescent microscopy 16 hours post transfection (−DHT), 4 hours after treatment (+DHT) with 1.0 nM DHT for GFP-AR and GFP-DBDH-LBD or 100 nM DHT for GFP-LBD, or 12 hours after androgen withdrawal (Withdrawal). The experiment was reproduced 5 times.