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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2014 Mar 22;0:473–480. doi: 10.1016/j.jsbmb.2014.03.004

Figure 2. Subcellular distribution of GFP-AR, GFP-DBDH-LBD and GFP-LBD in C4-2 cells.

Figure 2

C4-2 cells were transiently transfected with GFP-AR, GFP-DBDH-LBD and GFP-LBD and stained with Hoechst. The subcellular localization (A) and quantification (B) were assessed in androgen-free conditions by fluorescent microscopy 16 hours after transfection. The results are from five transfections for each experimental group. At least 200 cells were counted for each transfection to determine the percentage of cells displaying cytoplasmic (C), even (E), or nuclear (N) localization. The experiment was reproduced 5 times.