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. Author manuscript; available in PMC: 2015 Jun 4.
Published in final edited form as: Oncogene. 2014 Feb 10;33(49):5626–5636. doi: 10.1038/onc.2013.506

Fig. 3. MAF regulates glial differentiation markers and the neural crest marker SOX9.

Fig. 3

(A) RT-PCR analysis of S462TY MPNST cells transduced with mCherry tagged MAF or mCherry control for 48 hours. mRNA levels are expressed relative to β-actin. (B) Immohistochemistry shows expression of differentiation markers (S100β, MBP, and BLBP) in MAF transduced MPNST cells. (C) RT-PCR and western blot analysis showing relative expression of SOX9 in MAF transduced MPNST cells before (white bar) and after (black bar) induction with doxycycline for 48 hours. (D) ChIP analysis of MAF occupancy of the SOX9 promoter.