Effect of AMR-Me on regulation of metastasis-related signaling network in B16F10 cells. (A) Western blot analyses showing reduced expression of VEGF, FAK, pFAK397, JNK, pJNK, ERK1/2, pERK1/2 compared to that of the control cells by treatment with AMR-Me (5 μM) for 48 h. (B) The respective band intensities of the blots as calculated by Image J Launcher (version 1.4.3.67) reflected similar profile of significant inhibition in expression of VEGF, pFAK397, pJNK, pERK1/2 (P<0.01) as well as that of FAK, JNK and ERK1/2 (P<0.05) with AMR-Me (5 μM) for 48 h compared to vehicle control. (C) Graphical representation of ELISA showing significant reduction of VEGF, pFAK397, pJNK, pERK1/2 (*P<0.01) as well as that of FAK, JNK and ERK1/2 (P<0.05) with AMR-Me (5 μM) for 48 h with respect to vehicle control.