Table 2.
Activities of thioredoxin, thioredoxin reductase and GSSG reductase in heart mitochondria of youn][g adult and elderly Fischer 344 rats.
| Activity |
SSM |
IFM |
||
|---|---|---|---|---|
| 6-mo | 24-mo | 6-mo | 24-mo | |
| Trx | 0.38±0.04 | 0.46±0.04 | 0.45±0.07 | 0.46±0.04 |
| Trx reductase | 10.2±1.9 | 11.3±0.7 | 9.9±1.7 | 9.6±1.1 |
| GSSG reductase | 7.4±0.9 | 8.9±1.4 | 8.9±1.6 | 9.2±0.6 |
The activities of thioredoxin (Trx), thioredoxin reductase and GSSG reductase were measured in the SSM and IFM from both young adult (6–8 mo) and elderly (24–28 mo) F344 rat hearts, as described under Experimental procedures section. Values represent the mean±SEM for duplicate assays of at least three separate biological samples (n>3) and are expressed as nmol/min/mg of mitochondrial protein.