Table 2. The hyperboloid parameters for FBPase and ATCase derived by the nonlinear fitting method.
| FBPase | AVG(stdv) a (largest) | AVG(stdv) b (medium) | AVG(stdv) c (smallest) | trials used In estimation* |
| T state (1rdz) | 179.85(0.08) | 57.23(0.04) | 55.67(0.01) | 5 |
| R state (1cnq) | 189.28(0.08) | 56.47(0.04) | 56.19(0.01) | 5 |
| Difference | -9.43 | 0.76 | -0.52 | |
| % change | -4.98 | 1.33 | -0.92 | |
| ATCase | ||||
| ASP domain | AVG(stdv) a (largest) | AVG(stdv) b (medium) | AVG(stdv) c (smallest) | trials used in estimation* |
| T state (1za1) | 88.28(0.05) | 49.35(0.18) | 31.59(0.03) | 4 |
| R state (1d09) | 83.59(0.05) | 48.51(0.35) | 31.48(0.06) | 4 |
| Difference | 4.69 | 0.84 | 0.10 | |
| % change | 5.31 | 1.70 | 0.03 | |
| CP domain | ||||
| T state (1za1) | 132.91(0.02) | 56.05(0.26) | 39.91(0.06) | 4 |
| R state (1d09) | 132.10(0.03) | 57.15(0.02) | 39.39(0.01) | 4 |
| Difference | 0.81 | -1.10 | 0.52 | |
| % change | 0.60 | -1.96 | 1.30 | |
| reg domain | ||||
| T state (1za1) | 261.54(0.01) | 90.88(0.01) | 73.87(0.01) | 4 |
| R state (1d09) | 257.19(0.01) | 95.34(0.14) | 71.90(0.05) | 4 |
| Difference | 4.35 | -4.46 | 1.96 | |
| % change | 1.66 | -4.90 | 2.66 |
*
In most cases, the range of error was rather small, so that numerous runs were unnecessary. We determined heuristically that 4–6 calculations were sufficient to obtain reliable parameter values. Convergence was reached with an F value of 0.0054 where F=fi+1–fi is the convergence between cycles when improving the fit