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. Author manuscript; available in PMC: 2014 Dec 19.
Published in final edited form as: Nat Commun. 2014 Jun 19;5:4196. doi: 10.1038/ncomms5196

Figure 6. Perivascular glioma cells do not compromise vascular smooth muscle cell function.

Figure 6

DIC images of vessels without (top) or with (bottom) perivascular glioma cells before and after application of 125 nM U46619. Perivascular glioma cell presence was verified by eGFP-fluorescence (a). Average change in vessel diameters observed at high (h) (95%) and low (l) (20%) oxygen for vessels associated (grey) and not associated (black) with glioma cells when exposed to 125 nM U46619, 60 mM [K+]o, 10 nM ET-1 (b) and 1 μM PGE2 (c). For 1 μM PGE2, arterioles were preconstricted with 125 nM U46619 for 20 min. Statistical data as follows: 20% Oxygen. 125 nM U46619: (+)Tumor Cell(s) n=11, (-)Tumor Cell(s) n=11, two-tailed Mann Whitney Test, p=0.40; 60 mM [K+]o: (+)Tumor Cell(s) n=10, (-)Tumor Cell(s) n=9, two-tailed unpaired t-test, p=0.57; 10 nM ET-1: (+)Tumor Cell(s) n=8, (-)Tumor Cell(s) n=9, two-tailed unpaired t-test, p=0.78; 1 μM PGE2: (+)Tumor Cell(s) n=14, (-)Tumor Cell(s) n=21, two-tailed unpaired t-test, p=0.72. 95% Oxygen. 125 nM U46619: (+)Tumor Cell(s) n=14. (-)Tumor Cell(s) n=12, two-tailed unpaired t-test, p=0.99; 60 mM [K+]o: (+)Tumor Cell(s) n=11, (-)Tumor Cell(s) n=19, two-tailed Mann-Whitney Test, p>0.9999; 10 nM ET-1: (+)Tumor Cell(s) n=7, (-)Tumor Cell(s) n=9, two-tailed unpaired t-test, p=0.48). Error bars refer to SEM Scale, 20 μm.

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