Table 2. Kinetic parameters of the wild-type and mutant OLEI01171 proteins.
The salts used were 0.4 M NaCl and 0.1 M KCl.
| Protein | Variable substrate | Km (mM) | kcat (s−1) |
kcat/Km (s−1 · M−1) |
|---|---|---|---|---|
| Wild-type | α-Naphthyl acetate (no salt) | 0.40 ± 0.03 | 0.8 ± 0.02 | 0.2×104 |
| Wild-type | α-Naphthyl acetate (+NaCl) | 0.70 ± 0.04 | 3.3 ± 0.1 | 0.5×104 |
| Wild-type | α-Naphthyl acetate (+KCl) | 1.2 ± 0.1 | 6.5 ± 0.2 | 0.6×104 |
| Wild-type | α-Naphthyl propionate (+NaCl) | 0.20 ± 0.03 | 1.5 ± 0.1 | 0.8×104 |
| Wild-type | α-Naphthyl butyrate (+NaCl) | 0.20 ± 0.04 | 0.06 ± 0.01 | 0.3×103 |
| Wild-type | pNP-acetate (no salt) | 0.7 ± 0.1 | 0.2 ± 0.03 | 0.3×103 |
| Wild-type | pNP-acetate (+NaCl) | 1.0 ± 0.2 | 0.8 ± 0.1 | 0.2×103 |
| Wild-type | pNP-propionate (+NaCl) | 0.7 ± 0.2 | 0.30 ± 0.03 | 0.4×103 |
| Wild-type | pNP-butyrate (+NaCl) | 0.9 ± 0.3 | 0.20 ± 0.03 | 0.2×103 |
| C57A | α-Naphthyl acetate (+NaCl) | 0.20 ± 0.01 | 0.90 ± 0.02 | 0.5×104 |
| S58A | α-Naphthyl acetate (+NaCl) | 0.40 ± 0.01 | 0.8 ± 0.1 | 0.2×104 |
| N61A | α-Naphthyl acetate (+NaCl) | 0.5 ± 0.1 | 1.8 ± 0.2 | 0.4×104 |
| K65A | α-Naphthyl acetate (+NaCl) | 0.40 ± 0.01 | 1.2 ± 0.1 | 0.3×104 |
| Y97A | α-Naphthyl acetate (+NaCl) | 1.7 ± 0.3 | 2.9 ± 0.3 | 0.2×104 |
| S172A | α-Naphthyl acetate (+NaCl) | 0.6 ± 0.1 | 5.3 ± 0.3 | 0.9×104 |
| A223K | α-Naphthyl acetate (+NaCl) | 0.30 ± 0.04 | 1.1 ± 0.1 | 0.4×104 |
| N225A | α-Naphthyl acetate (+NaCl) | 0.5 ± 0.1 | 1.7 ± 0.1 | 0.3×104 |
| N225K | α-Naphthyl acetate (+NaCl) | 0.4 ± 0.1 | 3.0 ± 0.2 | 0.8×104 |