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The “no adjuvant used” model was created by intraperitoneally immunizing with only Mp antigen twice, on days −28 and −21 (for the RI, repeated inoculation, group) or without pre-treatment (for the SI, single inoculation, group), followed by intratracheal challenge with Mp antigen at day 0 for both groups. Those two models were evaluated at days 0, 1 2, 3, 4, and 7 by examination of bronchoalveolar lavage fluid or lung pathology.
