Table 3.
Primers and TaqMan probes for relative quantification with Comparative CT method.
| DEG | Primers sequence |
TaqMan probe sequence | |
|---|---|---|---|
| DEG1 | Forward: Reverse: |
5′-GGGCGTGTGCACAGAAG-3′ 5′-AAGTCCTCCTGAGTGACATGGA-3′ |
5′-CTCGCAGGGCATACAT-3′ |
| DEG2 | Forward: Reverse: |
5′-GATGCTTACCGAATCCGGAGATC-3′ 5′-GCATTGCTCTCTCACTGTTGTTAG-3′ |
5′-CCTCTTCCTCTTCCTCCTCC-3′ |
| DEG3 | Forward: Reverse: |
5′-CAACCGTCATTGGGTACAAAGG-3′ 5′-TGTAAGGGTCCAGCTGATCAAGA-3′ |
5′-ATGGCAAGAAAATCAC-3′ |
| DEG4 | Forward: Reverse: |
5′-CGGCCAGGAAACTTGAACTTG-3′ 5′-CCGAGCTGCAGAACAAGGA-3′ |
5′-CAGGGCCTCAATCACA-3′ |
| DEG5 | Forward: Reverse: |
5′-CTGGTCGAATGAGGCACCTAAAA-3′ 5′-TGGGTTTAGGTGTTGTTCCTTCAC-3′ |
5′-CATGCCTGAATCTGC-3′ |
| DEG6 | Forward: Reverse: |
5′-AGATTAGCGGGATGAAAACGTCTT-3′ 5′-CGCCCAGATGCCGAGAAAA-3′ |
5′-CCCCGTGATTGTTTTC-3′ |
| DEG7 | Forward: Reverse: |
5′-GGTAGTGATGTGCAAGAGTCCAT-3′ 5′-CCGCAGCGAGGAGTTTCT-3′ |
5′-CATTGCTGGAAAACTG-3′ |
| DEG8 | Forward: Reverse: |
5′-GAAGGAGACCATCAAAGGATTCCA-3′ 5′-GAAGGCCTGTTCTGGGAGATG-3′ |
5′-ATTCACCTGCCAAAATC-3′ |
| DEG9 | Forward: Reverse: |
5′-GGCAGGGTGGTCCTGAGA-3′ 5′-CCGCCATTGGCCTTAACTG-3′ |
5′-CCTCTCTCCGCCCCGGACA-3′ |
| DEG11 | Forward: Reverse: |
5′-CAGGTTTCAGTGAAGCCATCTG-3′ 5′-GGGTTGGCATCTACGTGTGA-3′ |
5′-CACCCAAGGGTAACAAC-3′ |
| DEG12 | Forward: Reverse: |
5′-CCAGGTCAAACTTGTGGATCCT-3′ 5′-GCTTCAGTAAATCTCCACTCGATCT-3′ |
5′-ATGGACAGGAAACCCAC-3′ |
| DEG14 | Forward: Reverse: |
5′-CCCGCTCCTTATCTGCAAGTT-3′ 5′-TCAAGATGGACGTGCACATTACTC-3′ |
5′-CATGCAGTGAACAAGC-3′ |
| DEG15 | Forward: Reverse: |
5′-CGGCCTCCAAGCTCTCT-3′ 5′-TGAGAACACGGGCAATGGATTT-3′ |
5′-CCGGACGACTCGGATCT-3′ |
| DEG16 | Forward: Reverse: |
5′-GGACTCTTCTGCTAATCGATGAACA-3′ 5′-GCCTCAACTTCGTCTGGAGAAAA-3′ |
5′-CAGATGGACCAATAAGTCA-3′ |
DEG, differentially expressed gene.