Skip to main content
. 2014 Aug 11;8:228. doi: 10.3389/fncel.2014.00228

FIGURE 8.

FIGURE 8

Bregs decrease infarct volumes in male PD-L2-/- mice, but are dispensable when transferred to PD-L1-/- mice 4 h after MCAO. (A) Intravenous transfer of 5 million IL10+ B-cells 4 h after surgery to induce MCAO in PD-L1-/- mice 96 h following 60 min of MCAO compared to intravenous transfer of RPMI vehicle (no cells) and its representative 2,3,5-triphenyltetrazolium chloride (TTC) stained cerebral sections 96 h following 60 min of MCAO. (B) Intravenous transfer of 5 million IL10+ B-cells 4 h after surgery to induce MCAO in PD-L2-/- mice 96 h following 60 min of MCAO compared to intravenous transfer of RPMI vehicle (no cells) and its representative TTC stained cerebral sections 96 h following 60 min of MCAO. Significance values represent mean ± SEM. *p < 0.05, **p < 0.01. Splenocytes from sham-treated and MCAO-subjected WT, PD-L1-/- and PD-L2-/- mice were harvested 96 h after MCAO (60 min) and assessed for expression of: (C) CD1dhiCD5+CD19+ (Breg) cells and (D) IL-10 production by gated Breg cells. Data are representative of two independent experiments with spleens processed from four to five individual mice (mean ± SEM). Significant differences between sample means are indicated (*p ≤ 0.05 compared to the PD-L1-/- mice, post MCAO). Significant differences within the strains are indicated (#p ≤ 0.05 and ###p ≤ 0.001 as compared to its respective sham-treated counterparts).