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. Author manuscript; available in PMC: 2014 Aug 11.
Published in final edited form as: Proteomics Clin Appl. 2013 May 17;7(0):378–383. doi: 10.1002/prca.201200062

Figure 2.

Figure 2

Optimization of the plasma screening protocol by blocking plasma samples with E.coli lysate and using HeLa IVTT expression system. A. Blocking plasma with E.coli lysate before probing onto arrays could reduce background signals. Two identical arrays were probed with the same plasma sample. Left, plasma pre-blocked by E.coli lysate; right, no pre-blocking. Plots of antibody response signal intensity of all protein spots are shown on the right: top, plasma pre-blocked by E.coli lysates; bottom, no pre-blocking. B. HeLa IVTT improved the detection of antibody response. Zoomed-in images of antibody response to EBNA on NAPPA arrays expressed by either Rabbit IVTT or HeLa IVTT are shown on the left. Experiment condition was the same as that in Figure 2A, with plasma pre-blocked by E.coli lysate. Signal to noise ratio plot of EBNA antibody responses is shown on the right. C. Plots of antibody response signal intensity of all protein spots. Increased autoantibody responses against several human proteins when using HeLa IVTT expressed NAPPA arrays were detected (shown in red dots).