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. Author manuscript; available in PMC: 2014 Aug 11.
Published in final edited form as: Obesity (Silver Spring). 2011 Aug 4;20(1):40–47. doi: 10.1038/oby.2011.259

Figure 2.

Figure 2

Indirect coculture of macrophages with adipocytes promotes adipocyte mRNA expression of inflammatory factors. Quantitative reverse transcriptase (RT)-PCR was performed using adipocyte mRNA. Adipocyte mRNA was isolated with and without prior indirect coculture with THP-1 macrophages, with and without costimulation using lipopolysaccharide (LPS) (10 ng/ml). mRNA expression of 3T3-L1-expressed IL-6, Mcp-1, and Rantes was enhanced with macrophage coculture and further enhanced by costimulation with LPS. Values represent the means of at least three independent experiments with s.e.m. *P < 0.05.