Figure 3.
Dexamethsone, tumor necrosis factor-α (TNFα), and forskolin stimulate zinc finger protein 36 (Zfp36) expression in 3T3-L1 adipocytes. Western blot analysis of Zfp36 expression in 3T3-L1 adipocytes grown in serum-free medium with the following 6 h treatments: vehicle control, TNFα (20 ng/ml), retinoic acid (RA, 1.0 μmol/l), dexamethasone (Dex, 1 μmol/l), fetal calf serum (FCS, 10%), and forskolin (10 μmol/l). Quantitative image analysis indicating Zfp36 expression relative to control cells, normalized to actin, is shown. Data are representative of three independent experiments.