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. Author manuscript; available in PMC: 2014 Aug 11.
Published in final edited form as: Obesity (Silver Spring). 2011 Aug 4;20(1):40–47. doi: 10.1038/oby.2011.259

Figure 6.

Figure 6

Forskolin and retinoic acid (RA), but not dexamethasone, rapidly enhance zinc finger protein 36 (Zfp36) expression in RAW 264.7 macrophages. (a) Western blot analysis of Zfp36 expression in RAW 264.7 cells grown in serum-free medium with the following 6 h treatments: vehicle control, lipopolysaccharide (LPS) (10 ng/ml), dexamethasone (Dex, 1 μmol/l), forskolin (Forsk, 10 μmol/l), and retinoic acid (RA, 1.0 μmol/l). Actin loading control is shown. Data are representative of three independent experiments. (b) Quantitative image analysis of western blots showing Zfp36 expression in RAW 264.7 cells after the same treatments described above. Band intensities were measured and normalized to respective Actin band intensities for three independent experiments. Data are shown relative to control cells. *P< 0.05 compared with control cells.