Figure 8.
Zinc finger protein 36 (Zfp36) reduces adipokine mRNA expression in 3T3-L1 adipocytes. Cells were transduced with empty vector-containing lentiviral particles (vector) or human Zfp36-expressing lentiviral particles as described in Figure 7. Quantitative reverse transcriptase (RT)-PCR was performed and expression was normalized to vector-treated cells (dashed line). mRNA expression of IL-6, Mcp-1, and IL-1β was reduced in Zfp36-expressing adipocytes (black bars). Values represent the means of three independent experiments with s.e.m. *P < 0.05 relative to vector control.