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. Author manuscript; available in PMC: 2014 Aug 11.
Published in final edited form as: Nat Chem Biol. 2012 Jul 29;8(9):784–790. doi: 10.1038/nchembio.1038

Figure 6. Peroxidasin is critical for collagen IV and basement membrane integrity.

Figure 6

(a) Confocal fluorescence microscopy images of Drosophila anterior midgut using a collagen IV GFP protein trap line (Viking-GFP) to delineate collagen IV distribution. Representative sections from wildtype Pxn +/+ (Pxn +/+), heterozygote Pxn +/− (Pxn+/f07229), and mutant Pxn −/− (Pxnf07229/f07229) flies are shown. Distorted and torn collagen IV networks (arrows) with gross defects (“holes”) in the circumferential muscle layer (asterisks) typified Pxn −/− sections. Scale bar = 10 μm. (b) Immunoblot of collagenase solubilized basement membrane isolated from Drosophila Pxn +/− and Pxn −/− larvae. Pxn −/− mutants demonstrate grossly reduced collagen IV immunoreactivity at 20.4% of wildtype, while Pxn +/− flies have relatively maintained collagen IV NC1 content at 82% of wildtype (Supplementary Fig. 11). Pxn −/− mutants also demonstrated a shift in the % uncross-linked immunoreactivity with 42% of total band density in the uncross-linked form compared to < 9% total band density in Pxn +/− flies (Supplementary Fig. 11).

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