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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Virology. 2014 Jul 16;0:351–362. doi: 10.1016/j.virol.2014.06.001

Figure 3. Analysis of sg mRNAs produced by MA104 cells infected with SHFVic virus.

Figure 3

(A) Northern blot analysis of viral sg mRNAs. MA104 cells were mock infected (M) or infected with SHFVic virus at an MOI of 1 and total RNA was extracted at 4, 8 16 or 24 h after infection. Cell RNA (1 μg) was electrophoresed on a 1% denaturing agarose gel, transferred to an Hybond-N+ membrane and hybridized with a DIG-labeled 5′ leader RNA probe. The sg mRNAs were identified based on size. (B) Top-Diagram indicating the positions of the RT-PCR primers used. Bottom- Cell RNA was extracted from MA104 cells 24 h after infection with SHFVic virus at an MOI of 1 and subjected to RT-PCR. The PCR products were separated on a 2% agarose gel. The ~1000 and ~500 bp DNA bands were excised and the extracted DNA was TA cloned and sequenced. (C) The junction sequence for sg mRNA 3′ obtained from the ~500 bp band was aligned with the genome and 5′ leader sequences.

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